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产品名称:CST代理P-P38 MAPK 抗体antibody(28B10)

产品型号:9216

产品报价:12

产品特点:CST代理P-P38 MAPK 抗体antibody(28B10)
CST spho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
Isotype:Mouse IgG1
Applications:W IP IF-IC F
Reactivity:H M R Mk Sc (Z)
Sensitivity:Endogenous
MW (kDa):43

9216CST代理P-P38 MAPK 抗体antibody(28B10)的详细资料:

CST代理P-P38 MAPK 抗体antibody(28B10) 

Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

9216:

Flow, Immunofluorescence, Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb detects p38 MAPK only when activated by dual phosphorylation at Thr180 and Tyr182. This antibody does not significantly cross-react with the corresponding phosphorylated forms of either p44/42 MAPK (Erk1/2) or SAPK/JNK. It does not detect nonphosphorylated p38 MAPK.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAPK.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (upper) or p38 MAPK Antibody #9212 (lower).

Flow Cytometry

Flow Cytometry

澳门百家必胜方法Flow cytometric analysis of Jurkat cells, untreated (green) or anisomycin-treated (blue), using Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells either untreated (left) or anisomycin treated (right) labeled with Phospho-p38 MAP Kinase (Thr180/Tyr182) (28B10) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor Phalloidin 555 (red).

CST代理P-P38 MAPK 抗体antibody(28B10) 

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